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recombinant denv 2 ns1 antigen (Native Antigen Inc)

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Native Antigen Inc recombinant denv 2 ns1 antigen
Recombinant Denv 2 Ns1 Antigen, supplied by Native Antigen Inc, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dengue+virus+ns1+protein+serotypes+1-4/Dengue+Virus+NS1+Protein+Serotypes+1-4/pm41833174-91-12-17
Average 94 stars, based on 13 article reviews

recombinant denv 2 ns1 antigen - by Bioz Stars, 2026-09

94/100 stars

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Article Title: Anti-dengue virus antibodies that elicit complement-mediated lysis of Zika virion correlate with protection from severe dengue disease.
Article Snippet: >0.5. Neutralizing antibody titers were measured against the heterotypic incoming serotype of each participant. Recombinant E (E) and NS1 proteins from DENV1-4 and ZIKV were commercially acquired from Native Antigen Co. (United Kingdom, DENV1-E #REC31679, DENV2-E #REC31680, DENV3-E #REC31681, DENV4-E #REC31682, ZIKV-E #ZIKVSU-ENV, DENV1-4-NS1 #DENVX4-NS1, ZIKV-NS1 #ZIKVSU-NS1) and used for biophysi

Plasmid Preparation:

Virus:

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Figure 1. Scheme of nanobody-based sandwich lateral flow. When the ZIKV NS1 protein antigen is added, it initially forms a complex with the AuNP- conjugated ZIKV_NbD6. This antigen-nanobody-AuNP complex is then immobilized on the test line by the capture nanobody ZIKV_Nb32. Any remaining nanobody-AuNP complexes are subsequently immobilized on the control line by an anti-VHH antibody. The LFA is considered positive when both the test and control lines are visible to the naked eye. It is negative when only the control line is visible. The assay is invalid if the control line is not visible.

Journal: ACS synthetic biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Figure 1. Scheme of nanobody-based sandwich lateral flow. When the ZIKV NS1 protein antigen is added, it initially forms a complex with the AuNP- conjugated ZIKV_NbD6. This antigen-nanobody-AuNP complex is then immobilized on the test line by the capture nanobody ZIKV_Nb32. Any remaining nanobody-AuNP complexes are subsequently immobilized on the control line by an anti-VHH antibody. The LFA is considered positive when both the test and control lines are visible to the naked eye. It is negative when only the control line is visible. The assay is invalid if the control line is not visible.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1−100).

Techniques: Control

Figure 4. Characterization of Zika NS1 by mass photometry. (A) The contrast distribution of Zika NS1 single molecule collisions at varying concentrations (25, 50, 100, and 250 nM) indicated the presence of oligomeric species at higher concentrations. (B) Apparent molecular weights (±1 SD) deduced from contrast distribution peaks in A are plotted against NS1 (monomer) concentration (Mr of monomer = 43.5 kDa). The expected Mr for different oligomers (monomer Mr = 43.5 kDa) are shown as broken lines with shading indicating the average standard deviation (expected accuracy) from contrast distribution peaks in that size range.

Journal: ACS synthetic biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Figure 4. Characterization of Zika NS1 by mass photometry. (A) The contrast distribution of Zika NS1 single molecule collisions at varying concentrations (25, 50, 100, and 250 nM) indicated the presence of oligomeric species at higher concentrations. (B) Apparent molecular weights (±1 SD) deduced from contrast distribution peaks in A are plotted against NS1 (monomer) concentration (Mr of monomer = 43.5 kDa). The expected Mr for different oligomers (monomer Mr = 43.5 kDa) are shown as broken lines with shading indicating the average standard deviation (expected accuracy) from contrast distribution peaks in that size range.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1−100).

Techniques: Concentration Assay, Standard Deviation

Figure 3. Binding analysis of ZIKV_Nb32 and ZIKV_NbD6 to the Zika NS1 antigen using BLI. Data were fit with a 1:1 binding model. (A) GST- tagged nanobodies immobilized on anti-GST sensors were exposed to a 2-fold dilution series of Zika NS1 starting at 100 nM. (B) NS1 target immobilized on Ni-NTA sensors exposed to the same GST-tagged nanobodies provided in the solution. The 2-fold dilution series started from 100 nM (Nb32) or 12.5 nM (NbD6). (C) Ni-NTA-immobilized NS1 exposed to StrepTag-II versions of both nanobodies using the same dilutions as in (B). The 13 nM trace was excluded as an outlier from the analysis of Nb32 (not shown). Structures of nanobodies are based on AlphaFold3 models.46 The NS1 tetramer and GST structures were taken from PDB (8WBG and 1GNW, respectively).

Journal: ACS synthetic biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Figure 3. Binding analysis of ZIKV_Nb32 and ZIKV_NbD6 to the Zika NS1 antigen using BLI. Data were fit with a 1:1 binding model. (A) GST- tagged nanobodies immobilized on anti-GST sensors were exposed to a 2-fold dilution series of Zika NS1 starting at 100 nM. (B) NS1 target immobilized on Ni-NTA sensors exposed to the same GST-tagged nanobodies provided in the solution. The 2-fold dilution series started from 100 nM (Nb32) or 12.5 nM (NbD6). (C) Ni-NTA-immobilized NS1 exposed to StrepTag-II versions of both nanobodies using the same dilutions as in (B). The 13 nM trace was excluded as an outlier from the analysis of Nb32 (not shown). Structures of nanobodies are based on AlphaFold3 models.46 The NS1 tetramer and GST structures were taken from PDB (8WBG and 1GNW, respectively).

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1−100).

Techniques: Binding Assay

Figure 5. Competitive BLI assay for (A) binding of NbD6 alone (replicated from Figure 3C) and (B) binding of NbD6 after sensor saturation with Nb32. Results were fit to a 1:1 binding model. Zika NS1 was immobilized on Ni-NTA sensors as before. NbD6 was applied in varying concentrations (2-fold serial dilution starting from 100 nM). (C) representative sensor trace (50 nM NbD6) showing all the steps in the competitive assay. Structures of nanobodies are based on AlphaFold3 models.46 The NS1 tetramer and GST structures were taken from PDB (8WBG and 1GNW, respectively).

Journal: ACS synthetic biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Figure 5. Competitive BLI assay for (A) binding of NbD6 alone (replicated from Figure 3C) and (B) binding of NbD6 after sensor saturation with Nb32. Results were fit to a 1:1 binding model. Zika NS1 was immobilized on Ni-NTA sensors as before. NbD6 was applied in varying concentrations (2-fold serial dilution starting from 100 nM). (C) representative sensor trace (50 nM NbD6) showing all the steps in the competitive assay. Structures of nanobodies are based on AlphaFold3 models.46 The NS1 tetramer and GST structures were taken from PDB (8WBG and 1GNW, respectively).

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1−100).

Techniques: Binding Assay, Serial Dilution

Figure 7. LOD determination of ZIKV LFA in running buffer. Lane1−7: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 250 ng/mL, lane 3: 25 ng/mL, lane 4: 2.5 ng/mL, lane 5: 0.25 ng/mL. (A) Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. (B) Capture Nb: GST-Nb32, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA.

Journal: ACS synthetic biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Figure 7. LOD determination of ZIKV LFA in running buffer. Lane1−7: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 250 ng/mL, lane 3: 25 ng/mL, lane 4: 2.5 ng/mL, lane 5: 0.25 ng/mL. (A) Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. (B) Capture Nb: GST-Nb32, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1−100).

Techniques: Membrane

Figure 8. Validation of ZIKV LFA using Strep-tagged nanobodies in human serum (A) and bovine urine (B). Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Lane1−8: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 0.5 μg/mL, lane 3: 0.1 μg/mL, lane 4: 20 ng/mL, lane 5: 4 ng/mL, lane 6: 2 ng/mL, lane 7: 1 ng/mL, lane 8: 0.5 ng/mL.

Journal: ACS synthetic biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Figure 8. Validation of ZIKV LFA using Strep-tagged nanobodies in human serum (A) and bovine urine (B). Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Lane1−8: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 0.5 μg/mL, lane 3: 0.1 μg/mL, lane 4: 20 ng/mL, lane 5: 4 ng/mL, lane 6: 2 ng/mL, lane 7: 1 ng/mL, lane 8: 0.5 ng/mL.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1−100).

Techniques: Biomarker Discovery, Membrane

Figure 9. Dengue NS1 test in our LFA using Strep-tagged nanobodies. Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Sample 1: 8.5 μg/mL Dengue serotype 1 NS1 protein, sample 2: 26.7 μg/mL Dengue serotype 2 NS1 protein, sample 3: 15.6 μg/mL Dengue serotype 3 NS1 protein, sample 4: 11.0 μg/mL Dengue serotype 4 NS1 protein.

Journal: ACS synthetic biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection.

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Figure 9. Dengue NS1 test in our LFA using Strep-tagged nanobodies. Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Sample 1: 8.5 μg/mL Dengue serotype 1 NS1 protein, sample 2: 26.7 μg/mL Dengue serotype 2 NS1 protein, sample 3: 15.6 μg/mL Dengue serotype 3 NS1 protein, sample 4: 11.0 μg/mL Dengue serotype 4 NS1 protein.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1−100).

Techniques: Membrane

Scheme of nanobody-based sandwich lateral flow. When the ZIKV NS1 protein antigen is added, it initially forms a complex with the AuNP-conjugated ZIKV_NbD6. This antigen-nanobody-AuNP complex is then immobilized on the test line by the capture nanobody ZIKV_Nb32. Any remaining nanobody-AuNP complexes are subsequently immobilized on the control line by an anti-VHH antibody. The LFA is considered positive when both the test and control lines are visible to the naked eye. It is negative when only the control line is visible. The assay is invalid if the control line is not visible.

Journal: ACS Synthetic Biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Scheme of nanobody-based sandwich lateral flow. When the ZIKV NS1 protein antigen is added, it initially forms a complex with the AuNP-conjugated ZIKV_NbD6. This antigen-nanobody-AuNP complex is then immobilized on the test line by the capture nanobody ZIKV_Nb32. Any remaining nanobody-AuNP complexes are subsequently immobilized on the control line by an anti-VHH antibody. The LFA is considered positive when both the test and control lines are visible to the naked eye. It is negative when only the control line is visible. The assay is invalid if the control line is not visible.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1–100).

Techniques: Control

Binding analysis of ZIKV_Nb32 and ZIKV_NbD6 to the Zika NS1 antigen using BLI. Data were fit with a 1:1 binding model. (A) GST-tagged nanobodies immobilized on anti-GST sensors were exposed to a 2-fold dilution series of Zika NS1 starting at 100 nM. (B) NS1 target immobilized on Ni-NTA sensors exposed to the same GST-tagged nanobodies provided in the solution. The 2-fold dilution series started from 100 nM (Nb32) or 12.5 nM (NbD6). (C) Ni-NTA-immobilized NS1 exposed to StrepTag-II versions of both nanobodies using the same dilutions as in (B). The 13 nM trace was excluded as an outlier from the analysis of Nb32 (not shown). Structures of nanobodies are based on AlphaFold3 models. The NS1 tetramer and GST structures were taken from PDB ( 8WBG and 1GNW , respectively).

Journal: ACS Synthetic Biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Binding analysis of ZIKV_Nb32 and ZIKV_NbD6 to the Zika NS1 antigen using BLI. Data were fit with a 1:1 binding model. (A) GST-tagged nanobodies immobilized on anti-GST sensors were exposed to a 2-fold dilution series of Zika NS1 starting at 100 nM. (B) NS1 target immobilized on Ni-NTA sensors exposed to the same GST-tagged nanobodies provided in the solution. The 2-fold dilution series started from 100 nM (Nb32) or 12.5 nM (NbD6). (C) Ni-NTA-immobilized NS1 exposed to StrepTag-II versions of both nanobodies using the same dilutions as in (B). The 13 nM trace was excluded as an outlier from the analysis of Nb32 (not shown). Structures of nanobodies are based on AlphaFold3 models. The NS1 tetramer and GST structures were taken from PDB ( 8WBG and 1GNW , respectively).

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1–100).

Techniques: Binding Assay

Characterization of Zika NS1 by mass photometry. (A) The contrast distribution of Zika NS1 single molecule collisions at varying concentrations (25, 50, 100, and 250 nM) indicated the presence of oligomeric species at higher concentrations. (B) Apparent molecular weights (±1 SD) deduced from contrast distribution peaks in A are plotted against NS1 (monomer) concentration ( M r of monomer = 43.5 kDa). The expected M r for different oligomers (monomer M r = 43.5 kDa) are shown as broken lines with shading indicating the average standard deviation (expected accuracy) from contrast distribution peaks in that size range.

Journal: ACS Synthetic Biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Characterization of Zika NS1 by mass photometry. (A) The contrast distribution of Zika NS1 single molecule collisions at varying concentrations (25, 50, 100, and 250 nM) indicated the presence of oligomeric species at higher concentrations. (B) Apparent molecular weights (±1 SD) deduced from contrast distribution peaks in A are plotted against NS1 (monomer) concentration ( M r of monomer = 43.5 kDa). The expected M r for different oligomers (monomer M r = 43.5 kDa) are shown as broken lines with shading indicating the average standard deviation (expected accuracy) from contrast distribution peaks in that size range.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1–100).

Techniques: Concentration Assay, Standard Deviation

Competitive BLI assay for (A) binding of NbD6 alone (replicated from Figure C) and (B) binding of NbD6 after sensor saturation with Nb32. Results were fit to a 1:1 binding model. Zika NS1 was immobilized on Ni-NTA sensors as before. NbD6 was applied in varying concentrations (2-fold serial dilution starting from 100 nM). (C) representative sensor trace (50 nM NbD6) showing all the steps in the competitive assay. Structures of nanobodies are based on AlphaFold3 models. The NS1 tetramer and GST structures were taken from PDB ( 8WBG and 1GNW , respectively).

Journal: ACS Synthetic Biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Competitive BLI assay for (A) binding of NbD6 alone (replicated from Figure C) and (B) binding of NbD6 after sensor saturation with Nb32. Results were fit to a 1:1 binding model. Zika NS1 was immobilized on Ni-NTA sensors as before. NbD6 was applied in varying concentrations (2-fold serial dilution starting from 100 nM). (C) representative sensor trace (50 nM NbD6) showing all the steps in the competitive assay. Structures of nanobodies are based on AlphaFold3 models. The NS1 tetramer and GST structures were taken from PDB ( 8WBG and 1GNW , respectively).

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1–100).

Techniques: Binding Assay, Serial Dilution

LOD determination of ZIKV LFA in running buffer. Lane1–7: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 250 ng/mL, lane 3: 25 ng/mL, lane 4: 2.5 ng/mL, lane 5: 0.25 ng/mL. (A) Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. (B) Capture Nb: GST-Nb32, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA.

Journal: ACS Synthetic Biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: LOD determination of ZIKV LFA in running buffer. Lane1–7: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 250 ng/mL, lane 3: 25 ng/mL, lane 4: 2.5 ng/mL, lane 5: 0.25 ng/mL. (A) Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. (B) Capture Nb: GST-Nb32, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1–100).

Techniques: Membrane

Validation of ZIKV LFA using Strep-tagged nanobodies in human serum (A) and bovine urine (B). Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Lane1–8: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 0.5 μg/mL, lane 3: 0.1 μg/mL, lane 4: 20 ng/mL, lane 5: 4 ng/mL, lane 6: 2 ng/mL, lane 7: 1 ng/mL, lane 8: 0.5 ng/mL.

Journal: ACS Synthetic Biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Validation of ZIKV LFA using Strep-tagged nanobodies in human serum (A) and bovine urine (B). Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Lane1–8: dilution series of ZIKV NS1 protein. Lane 1: 2.5 μg/mL, lane 2: 0.5 μg/mL, lane 3: 0.1 μg/mL, lane 4: 20 ng/mL, lane 5: 4 ng/mL, lane 6: 2 ng/mL, lane 7: 1 ng/mL, lane 8: 0.5 ng/mL.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1–100).

Techniques: Membrane

Dengue NS1 test in our LFA using Strep-tagged nanobodies. Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Sample 1: 8.5 μg/mL Dengue serotype 1 NS1 protein, sample 2: 26.7 μg/mL Dengue serotype 2 NS1 protein, sample 3: 15.6 μg/mL Dengue serotype 3 NS1 protein, sample 4: 11.0 μg/mL Dengue serotype 4 NS1 protein.

Journal: ACS Synthetic Biology

Article Title: Nanobody-Based Lateral Flow Assay for Rapid Zika Virus Detection

doi: 10.1021/acssynbio.4c00819

Figure Lengend Snippet: Dengue NS1 test in our LFA using Strep-tagged nanobodies. Capture Nb: Nb32-Strep, detection Nb: NbD6-Strep. NC membrane blocked with 0.6% BSA. Sample 1: 8.5 μg/mL Dengue serotype 1 NS1 protein, sample 2: 26.7 μg/mL Dengue serotype 2 NS1 protein, sample 3: 15.6 μg/mL Dengue serotype 3 NS1 protein, sample 4: 11.0 μg/mL Dengue serotype 4 NS1 protein.

Article Snippet: All four of the Dengue NS1 target proteins were purchased from the Native Antigen Company (cat: DENVX4-NS1–100).

Techniques: Membrane

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recombinant denv 2 ns1 antigen (Native Antigen Inc)

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